Specimen SS-F-SWT-874 // SWEET · IFI-S
Mabinlin Expression Series V-AG-B
Saccharum officinarum
Sugarcane
Target Trait
7.5
IFI score
Mabinlin sweet protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-874
Genomic Edits
7
GC Content
43.6%
Construct Length
3,873 bp
Promoter System
35S CaMV promoter
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TAS1R2/TAS1R3
IFI Score
750
Pathway Edits
- MAB-II-codon codon-optimized mabinlin II expression cassette for heterologous production in sugarcane
- 35S enhancer-driven promoter element for constitutive high-level mabinlin II expression
- Signal peptide fusion for apoplastic targeting and enhanced mabinlin II protein stability
- BiP chaperone co-expression for correct mabinlin II disulfide bridge formation and folding
- Protease inhibitor PR-1 co-expression to protect accumulated mabinlin II from proteolysis
- mRNA stability element insertion for extended mabinlin II transcript half-life in sugarcane
- Protein stabilizing osmolyte accumulation element for enhanced mabinlin thermostability
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: industrial mabinlin production for scalable heat-stable sweet protein food ingredient manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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