Specimen SS-F-SWT-872 // SWEET · IFI-S
Mabinlin Expression Series IV-IV-B
Saccharum officinarum
Sugarcane
Target Trait
6.33
IFI score
Mabinlin sweet protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-872
Genomic Edits
6
GC Content
45.9%
Construct Length
3,628 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
633
Pathway Edits
- MAB-II-codon codon-optimized mabinlin II expression cassette for heterologous production in sugarcane
- 35S enhancer-driven promoter element for constitutive high-level mabinlin II expression
- Signal peptide fusion for apoplastic targeting and enhanced mabinlin II protein stability
- BiP chaperone co-expression for correct mabinlin II disulfide bridge formation and folding
- Protease inhibitor PR-1 co-expression to protect accumulated mabinlin II from proteolysis
- mRNA stability element insertion for extended mabinlin II transcript half-life in sugarcane
- Protein stabilizing osmolyte accumulation element for enhanced mabinlin thermostability
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: regulatory sweet protein documentation for mabinlin-based thermostable natural sweetener filings with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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