IFI-S · Mabinlin Expression Series

Specimen SS-F-SWT-856 // SWEET · IFI-S

Mabinlin Expression Series II-IV-B

Saccharum officinarum

Sugarcane

Tier IIMabinlin Expression Series

Target Trait

3.97

IFI score

Mabinlin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-856

Genomic Edits

4

GC Content

43.7%

Construct Length

2,734 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

397

Pathway Edits

  • MAB-II-codon codon-optimized mabinlin II expression cassette for heterologous production in sugarcane
  • 35S enhancer-driven promoter element for constitutive high-level mabinlin II expression
  • Signal peptide fusion for apoplastic targeting and enhanced mabinlin II protein stability
  • BiP chaperone co-expression for correct mabinlin II disulfide bridge formation and folding
  • Protease inhibitor PR-1 co-expression to protect accumulated mabinlin II from proteolysis

Key Interventions

MAB-II codon-optimized transgene integration for high-yield mabinlin II sweet protein in sugarcaneSignal peptide engineering for apoplastic targeting and enhanced mabinlin II protein stability35S enhancer-driven transcriptional amplification for maximum mabinlin II expression output

IFI Subscale Profile

IFI Profile

IFI-S
397
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: pilot-scale mabinlin production for natural ultra-high-intensity thermostable sweetener formulation testing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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