IFI-S · Sucrose Synthase Accumulation Series

Specimen SS-F-SWT-840 // SWEET · IFI-S

Sucrose Synthase Accumulation Series V-IV-B

Capsicum annuum

Pepper

Tier VSucrose Synthase Accumulation Series

Target Trait

3×

fold-change

Sucrose pool fold-change

Technical Specifications

SKU

SS-F-SWT-840

Genomic Edits

6

GC Content

44.2%

Construct Length

3,515 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

300

Pathway Edits

  • SUS4 sucrose synthase isoform 4 overexpression for sucrose synthesis in Capsicum pericarp
  • SUS3 sucrose synthase isoform 3 co-expression for expanded sucrose biosynthetic capacity
  • SuSy sucrose synthase activity enhancement for increased sucrose accumulation in fruit tissue
  • INV-vac vacuolar invertase downregulation to prevent sucrose hydrolysis and maintain pool
  • SUT1 sugar transporter upregulation for phloem unloading and sink sucrose import enhancement
  • Hexokinase activity modulation for feedback-insensitive sucrose accumulation in pepper tissue

Key Interventions

SUS4 and SUS3 isoform co-overexpression expanding sucrose synthesis capacity in Capsicum fruitINV-vac vacuolar invertase downregulation preventing sucrose hydrolysis to maintain elevated poolSUT1 transporter upregulation enhancing phloem unloading for increased sink sucrose import

IFI Subscale Profile

IFI Profile

IFI-S
300
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: industrial sucrose pool engineering in pepper for large-scale naturally sweeter fruit ingredient supply with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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