IFI-S · Sucrose Synthase Accumulation Series
Specimen SS-F-SWT-807 // SWEET · IFI-S
Sucrose Synthase Accumulation Series I-IV-A
Capsicum annuum
Pepper
Tier ISucrose Synthase Accumulation Series
Target Trait
1.2×
fold-change
Sucrose pool fold-change
Technical Specifications
SKU
SS-F-SWT-807
Genomic Edits
3
GC Content
46%
Construct Length
2,490 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
120
Pathway Edits
- SUS4 sucrose synthase isoform 4 overexpression for sucrose synthesis in Capsicum pericarp
- SUS3 sucrose synthase isoform 3 co-expression for expanded sucrose biosynthetic capacity
- SuSy sucrose synthase activity enhancement for increased sucrose accumulation in fruit tissue
- INV-vac vacuolar invertase downregulation to prevent sucrose hydrolysis and maintain pool
Key Interventions
SUS4 and SUS3 isoform co-overexpression expanding sucrose synthesis capacity in Capsicum fruitINV-vac vacuolar invertase downregulation preventing sucrose hydrolysis to maintain elevated poolSUT1 transporter upregulation enhancing phloem unloading for increased sink sucrose import
IFI Subscale Profile
IFI Profile
IFI-S120
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
sucrose pool engineering research in Capsicum for natural sweetness enhancement mechanism characterization
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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