Steviol Glycoside Diversity Series III-BL-A

Stevia rebaudiana

Stevia

Tier IIISteviol Glycoside Diversity Series

Target Trait

3.3×

fold-change

Steviol glycoside diversity fold-change

Technical Specifications

SKU

SS-F-SWT-779

Genomic Edits

GC Content

46.5%

Construct Length

3,996 bp

Promoter System

Ubiquitin/35S CaMV promoter

Expression System

Biolistic particle bombardment

Host Strain

Gold particle He-bombardment

Receptor Target

TAS1R2/TAS1R3

IFI Score

330

Pathway Edits

UGT76G1-var variant library expression for expanded C-13 steviol glycoside glucosylation specificity
UGT74G1-var variant panel for diversified C-19 glucosylation producing RebD, RebE, RebF profiles
GT-library combinatorial glycosyltransferase panel for novel minor steviol glycoside synthesis
Directed UGT evolution cassette for engineered glycosylation regioselectivity toward rare rebaudiosides
RebD accumulation element via UGT91D2-enhanced cascade targeting specific C-3' glucosylation
RebE biosynthetic cassette integration for multi-glucosyl configuration toward elevated RebE content

Key Interventions

UGT76G1 and UGT74G1 variant library expression for expanded steviol glycoside diversity profileGT-library combinatorial panel for novel minor rebaudioside (D, E, F) biosynthesis in SteviaRebD-specific UGT91D2 enhancement for accumulation of the highest-quality minor glycoside

IFI Subscale Profile

IFI Profile

IFI-S

330

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

commercial diversified steviol glycoside platform for rare rebaudioside ingredient supply to premium markets

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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