Steviol Glycoside Diversity Series I-IV-B

Stevia rebaudiana

Stevia

Tier ISteviol Glycoside Diversity Series

Target Trait

1.8×

fold-change

Steviol glycoside diversity fold-change

Technical Specifications

SKU

SS-F-SWT-768

Genomic Edits

GC Content

45.2%

Construct Length

2,807 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

180

Pathway Edits

UGT76G1-var variant library expression for expanded C-13 steviol glycoside glucosylation specificity
UGT74G1-var variant panel for diversified C-19 glucosylation producing RebD, RebE, RebF profiles
GT-library combinatorial glycosyltransferase panel for novel minor steviol glycoside synthesis
Directed UGT evolution cassette for engineered glycosylation regioselectivity toward rare rebaudiosides

Key Interventions

UGT76G1 and UGT74G1 variant library expression for expanded steviol glycoside diversity profileGT-library combinatorial panel for novel minor rebaudioside (D, E, F) biosynthesis in SteviaRebD-specific UGT91D2 enhancement for accumulation of the highest-quality minor glycoside

IFI Subscale Profile

IFI Profile

IFI-S

180

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: steviol glycoside diversity research for minor rebaudioside characterization and quality assessment with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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