Fructan-to-Fructose Series V-IV-B

Saccharum officinarum

Sugarcane

Tier VFructan-to-Fructose Series

Target Trait

3.5×

fold-change

Fructose release fold-change

Technical Specifications

SKU

SS-F-SWT-760

Genomic Edits

GC Content

44.2%

Construct Length

3,935 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

350

Pathway Edits

FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue
Fructose transporter SWEET6 upregulation for efficient fructose distribution to consumer cells
Fructan biosynthesis FFT enzyme downregulation to shift balance toward hydrolysis
PHT phosphate transporter expression for cellular energy balance during inulin turnover

Key Interventions

FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue

IFI Subscale Profile

IFI Profile

IFI-S

350

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: industrial fructan exohydrolase engineering for large-scale natural fructose release from inulin reserves with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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