IFI-S · Fructan-to-Fructose Series

Specimen SS-F-SWT-759 // SWEET · IFI-S

Fructan-to-Fructose Series V-IV-A

Saccharum officinarum

Sugarcane

Tier VFructan-to-Fructose Series

Target Trait

3.5×

fold-change

Fructose release fold-change

Technical Specifications

SKU

SS-F-SWT-759

Genomic Edits

7

GC Content

47.4%

Construct Length

3,918 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

350

Pathway Edits

  • FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
  • FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
  • SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
  • INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue
  • Fructose transporter SWEET6 upregulation for efficient fructose distribution to consumer cells
  • Fructan biosynthesis FFT enzyme downregulation to shift balance toward hydrolysis
  • PHT phosphate transporter expression for cellular energy balance during inulin turnover

Key Interventions

FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue

IFI Subscale Profile

IFI Profile

IFI-S
350
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

industrial fructan exohydrolase engineering for large-scale natural fructose release from inulin reserves

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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