IFI-S · Fructan-to-Fructose Series
Specimen SS-F-SWT-757 // SWEET · IFI-S
Fructan-to-Fructose Series V-PR-A
Saccharum officinarum
Sugarcane
Tier VFructan-to-Fructose Series
Target Trait
3.5×
fold-change
Fructose release fold-change
Technical Specifications
SKU
SS-F-SWT-757
Genomic Edits
7
GC Content
46.9%
Construct Length
3,884 bp
Promoter System
35S CaMV promoter
Expression System
PEG-mediated protoplast transfection
Host Strain
PEG/calcium transfection
Receptor Target
TAS1R2/TAS1R3
IFI Score
350
Pathway Edits
- FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
- FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
- SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
- INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue
- Fructose transporter SWEET6 upregulation for efficient fructose distribution to consumer cells
- Fructan biosynthesis FFT enzyme downregulation to shift balance toward hydrolysis
- PHT phosphate transporter expression for cellular energy balance during inulin turnover
Key Interventions
FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue
IFI Subscale Profile
IFI Profile
IFI-S350
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
industrial fructan exohydrolase engineering for large-scale natural fructose release from inulin reserves
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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