IFI-S · Fructan-to-Fructose Series

Specimen SS-F-SWT-744 // SWEET · IFI-S

Fructan-to-Fructose Series III-IV-B

Saccharum officinarum

Sugarcane

Tier IIIFructan-to-Fructose Series

Target Trait

2.45×

fold-change

Fructose release fold-change

Technical Specifications

SKU

SS-F-SWT-744

Genomic Edits

5

GC Content

44.3%

Construct Length

3,241 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

245

Pathway Edits

  • FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
  • FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
  • SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
  • INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue
  • Fructose transporter SWEET6 upregulation for efficient fructose distribution to consumer cells
  • Fructan biosynthesis FFT enzyme downregulation to shift balance toward hydrolysis

Key Interventions

FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue

IFI Subscale Profile

IFI Profile

IFI-S
245
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: commercial fructan mobilization platform in sugarcane for enhanced natural fructose ingredient supply with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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