IFI-S · Fructan-to-Fructose Series
Specimen SS-F-SWT-743 // SWEET · IFI-S
Fructan-to-Fructose Series III-IV-A
Saccharum officinarum
Sugarcane
Tier IIIFructan-to-Fructose Series
Target Trait
2.45×
fold-change
Fructose release fold-change
Technical Specifications
SKU
SS-F-SWT-743
Genomic Edits
5
GC Content
48.9%
Construct Length
3,224 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
245
Pathway Edits
- FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
- FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
- SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
- INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue
- Fructose transporter SWEET6 upregulation for efficient fructose distribution to consumer cells
- Fructan biosynthesis FFT enzyme downregulation to shift balance toward hydrolysis
Key Interventions
FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue
IFI Subscale Profile
IFI Profile
IFI-S245
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
commercial fructan mobilization platform in sugarcane for enhanced natural fructose ingredient supply
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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