IFI-S · Fructan-to-Fructose Series

Specimen SS-F-SWT-734 // SWEET · IFI-S

Fructan-to-Fructose Series II-PR-B

Saccharum officinarum

Sugarcane

Tier IIFructan-to-Fructose Series

Target Trait

1.92×

fold-change

Fructose release fold-change

Technical Specifications

SKU

SS-F-SWT-734

Genomic Edits

4

GC Content

45.4%

Construct Length

2,860 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

192

Pathway Edits

  • FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
  • FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
  • SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
  • INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue
  • Fructose transporter SWEET6 upregulation for efficient fructose distribution to consumer cells

Key Interventions

FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue

IFI Subscale Profile

IFI Profile

IFI-S
192
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: pilot-scale fructan-to-fructose conversion for natural fructose-enriched sweetener production trials with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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