IFI-S · Fructan-to-Fructose Series

Specimen SS-F-SWT-726 // SWEET · IFI-S

Fructan-to-Fructose Series I-PR-B

Saccharum officinarum

Sugarcane

Tier IFructan-to-Fructose Series

Target Trait

1.4×

fold-change

Fructose release fold-change

Technical Specifications

SKU

SS-F-SWT-726

Genomic Edits

3

GC Content

44.7%

Construct Length

2,513 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

140

Pathway Edits

  • FEH-IIb fructan exohydrolase beta-IIb expression for inulin terminal fructose cleavage in sugarcane
  • FEH-IIa fructan exohydrolase alpha-IIa co-expression for branched fructan hydrolysis
  • SuSy sucrose synthase co-expression to maintain sink-tissue sucrose-fructose partitioning
  • INV1 vacuolar invertase expression for fructan hydrolysis complement in sink storage tissue

Key Interventions

FEH-IIb and FEH-IIa co-expression for comprehensive inulin-type fructan hydrolysis in sugarcaneSuSy co-expression maintaining sucrose pool balance while fructose is released from fructan reservesINV1 vacuolar invertase co-expression complementing fructan exohydrolase activity in storage tissue

IFI Subscale Profile

IFI Profile

IFI-S
140
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: fructan hydrolysis research in sugarcane for in-situ natural sweetness engineering from stored inulin with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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