IFI-S · Neo-Hesperidin Series

Specimen SS-F-SWT-704 // SWEET · IFI-S

Neo-Hesperidin Series III-IV-B

Capsicum annuum

Pepper

Tier IIINeo-Hesperidin Series

Target Trait

4.75×

fold-change

Neo-hesperidin dihydrochalcone fold-change

Technical Specifications

SKU

SS-F-SWT-704

Genomic Edits

7

GC Content

44.3%

Construct Length

3,921 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

475

Pathway Edits

  • CHS chalcone synthase overexpression for naringenin chalcone precursor flux in Capsicum
  • CHI chalcone isomerase upregulation for naringenin intermediate accumulation
  • F3H flavanone-3-hydroxylase activity enhancement for dihydrokaempferol pathway intermediate
  • NHDC-conv neohesperidin dihydrochalcone biosynthesis conversion cassette for chalcone reduction
  • CYP75B1 cytochrome P450 integration for methoxylation step in neohesperidin biosynthesis
  • PAL phenylalanine ammonia-lyase upregulation for expanded phenylpropanoid precursor flux

Key Interventions

CHS and CHI sequential overexpression driving naringenin flux for NHDC precursor productionNHDC-conv conversion cassette for catalyzing chalcone reduction toward neo-hesperidin dihydrochalconePAL upregulation expanding phenylpropanoid precursor availability for NHDC biosynthesis

IFI Subscale Profile

IFI Profile

IFI-S
475
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: commercial NHDC accumulation platform in pepper for plant-derived intense sweetener ingredient supply with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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