IFI-S · Trehalose Sweetness Series

Specimen SS-F-SWT-672 // SWEET · IFI-S

Trehalose Sweetness Series IV-IV-B

Saccharum officinarum

Sugarcane

Tier IVTrehalose Sweetness Series

Target Trait

2.38×

fold-change

Trehalose accumulation fold-change

Technical Specifications

SKU

SS-F-SWT-672

Genomic Edits

5

GC Content

45.9%

Construct Length

3,248 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

238

Pathway Edits

  • TPS1 trehalose-6-phosphate synthase overexpression for increased trehalose-6-P biosynthesis
  • TPS2 trehalose-6-phosphate phosphatase upregulation for trehalose-6-P to trehalose conversion
  • TPP trehalose phosphate phosphatase co-expression for complete trehalose accumulation support
  • PGM phosphoglucomutase activity optimization for expanded glucose-6-P precursor availability
  • Trehalase TRE1 downregulation to prevent trehalose catabolism and maintain high steady state
  • Vacuolar sequestration signal integration for trehalose storage and stress protection in sugarcane

Key Interventions

TPS1 and TPS2 sequential overexpression for complete trehalose-6-phosphate to trehalose conversionTPP co-expression ensuring efficient trehalose-6-P dephosphorylation for free trehalose accumulationTRE1 downregulation preventing trehalose catabolism to sustain elevated steady-state levels

IFI Subscale Profile

IFI Profile

IFI-S
238
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: regulatory metabolite documentation for trehalose-enriched sugarcane as a natural low-glycemic sweetener with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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