IFI-S · Trehalose Sweetness Series

Specimen SS-F-SWT-662 // SWEET · IFI-S

Trehalose Sweetness Series III-PR-B

Saccharum officinarum

Sugarcane

Tier IIITrehalose Sweetness Series

Target Trait

1.95×

fold-change

Trehalose accumulation fold-change

Technical Specifications

SKU

SS-F-SWT-662

Genomic Edits

5

GC Content

43.8%

Construct Length

3,247 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

195

Pathway Edits

  • TPS1 trehalose-6-phosphate synthase overexpression for increased trehalose-6-P biosynthesis
  • TPS2 trehalose-6-phosphate phosphatase upregulation for trehalose-6-P to trehalose conversion
  • TPP trehalose phosphate phosphatase co-expression for complete trehalose accumulation support
  • PGM phosphoglucomutase activity optimization for expanded glucose-6-P precursor availability
  • Trehalase TRE1 downregulation to prevent trehalose catabolism and maintain high steady state
  • Vacuolar sequestration signal integration for trehalose storage and stress protection in sugarcane

Key Interventions

TPS1 and TPS2 sequential overexpression for complete trehalose-6-phosphate to trehalose conversionTPP co-expression ensuring efficient trehalose-6-P dephosphorylation for free trehalose accumulationTRE1 downregulation preventing trehalose catabolism to sustain elevated steady-state levels

IFI Subscale Profile

IFI Profile

IFI-S
195
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: commercial trehalose platform in sugarcane for food-grade disaccharide sweetener ingredient supply with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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