IFI-S · Trehalose Sweetness Series

Specimen SS-F-SWT-653 // SWEET · IFI-S

Trehalose Sweetness Series II-PR-A

Saccharum officinarum

Sugarcane

Tier IITrehalose Sweetness Series

Target Trait

1.52×

fold-change

Trehalose accumulation fold-change

Technical Specifications

SKU

SS-F-SWT-653

Genomic Edits

4

GC Content

46.8%

Construct Length

2,883 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

152

Pathway Edits

  • TPS1 trehalose-6-phosphate synthase overexpression for increased trehalose-6-P biosynthesis
  • TPS2 trehalose-6-phosphate phosphatase upregulation for trehalose-6-P to trehalose conversion
  • TPP trehalose phosphate phosphatase co-expression for complete trehalose accumulation support
  • PGM phosphoglucomutase activity optimization for expanded glucose-6-P precursor availability
  • Trehalase TRE1 downregulation to prevent trehalose catabolism and maintain high steady state

Key Interventions

TPS1 and TPS2 sequential overexpression for complete trehalose-6-phosphate to trehalose conversionTPP co-expression ensuring efficient trehalose-6-P dephosphorylation for free trehalose accumulationTRE1 downregulation preventing trehalose catabolism to sustain elevated steady-state levels

IFI Subscale Profile

IFI Profile

IFI-S
152
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

pilot-scale trehalose production for low-glycemic natural sweetener with cryoprotective properties

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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