Specimen SS-F-SWT-640 // SWEET · IFI-S
Sorbitol-to-Sweetness Series V-IV-B
Saccharum officinarum
Sugarcane
Target Trait
3.2×
fold-change
Sorbitol accumulation fold-change
Technical Specifications
SKU
SS-F-SWT-640
Genomic Edits
7
GC Content
44.2%
Construct Length
3,895 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
320
Pathway Edits
- S6PDH sorbitol-6-phosphate dehydrogenase overexpression for direct conversion of glucose-6-P to sorbitol
- SDH sorbitol dehydrogenase downregulation to prevent catabolism of accumulated sorbitol
- SPS sucrose phosphate synthase activity optimization for sucrose-sorbitol metabolic balance
- SPP sucrose phosphate phosphatase co-expression for enhanced sucrose-to-sorbitol flux management
- Fructokinase activity suppression to redirect fructose toward the sorbitol biosynthetic route
- Vacuolar H+-ATPase upregulation for enhanced sorbitol compartmentalization in sugarcane
- SWEET transporter expression for optimized sorbitol loading into phloem and sink tissues
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: industrial sorbitol engineering in sugarcane for large-scale low-calorie polyol sweetener manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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