IFI-S · Sorbitol-to-Sweetness Series
Specimen SS-F-SWT-623 // SWEET · IFI-S
Sorbitol-to-Sweetness Series III-IV-A
Saccharum officinarum
Sugarcane
Tier IIISorbitol-to-Sweetness Series
Target Trait
2.25×
fold-change
Sorbitol accumulation fold-change
Technical Specifications
SKU
SS-F-SWT-623
Genomic Edits
5
GC Content
48.9%
Construct Length
3,184 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
225
Pathway Edits
- S6PDH sorbitol-6-phosphate dehydrogenase overexpression for direct conversion of glucose-6-P to sorbitol
- SDH sorbitol dehydrogenase downregulation to prevent catabolism of accumulated sorbitol
- SPS sucrose phosphate synthase activity optimization for sucrose-sorbitol metabolic balance
- SPP sucrose phosphate phosphatase co-expression for enhanced sucrose-to-sorbitol flux management
- Fructokinase activity suppression to redirect fructose toward the sorbitol biosynthetic route
- Vacuolar H+-ATPase upregulation for enhanced sorbitol compartmentalization in sugarcane
Key Interventions
S6PDH overexpression for direct glucose-6-phosphate conversion to sorbitol in sugarcaneSDH downregulation preventing sorbitol catabolism to maintain high steady-state accumulationSPS co-optimization balancing sucrose-sorbitol carbon partitioning for desired sweetness profile
IFI Subscale Profile
IFI Profile
IFI-S225
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
commercial sorbitol-enriched sugarcane platform for confectionery and pharmaceutical sweetener supply
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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