IFI-S · Sorbitol-to-Sweetness Series
Specimen SS-F-SWT-615 // SWEET · IFI-S
Sorbitol-to-Sweetness Series II-IV-A
Saccharum officinarum
Sugarcane
Tier IISorbitol-to-Sweetness Series
Target Trait
1.78×
fold-change
Sorbitol accumulation fold-change
Technical Specifications
SKU
SS-F-SWT-615
Genomic Edits
4
GC Content
47.3%
Construct Length
2,837 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
178
Pathway Edits
- S6PDH sorbitol-6-phosphate dehydrogenase overexpression for direct conversion of glucose-6-P to sorbitol
- SDH sorbitol dehydrogenase downregulation to prevent catabolism of accumulated sorbitol
- SPS sucrose phosphate synthase activity optimization for sucrose-sorbitol metabolic balance
- SPP sucrose phosphate phosphatase co-expression for enhanced sucrose-to-sorbitol flux management
- Fructokinase activity suppression to redirect fructose toward the sorbitol biosynthetic route
Key Interventions
S6PDH overexpression for direct glucose-6-phosphate conversion to sorbitol in sugarcaneSDH downregulation preventing sorbitol catabolism to maintain high steady-state accumulationSPS co-optimization balancing sucrose-sorbitol carbon partitioning for desired sweetness profile
IFI Subscale Profile
IFI Profile
IFI-S178
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
pilot-scale sorbitol production for natural low-calorie sweetener with cooling sensation formulation
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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