IFI-S · Sorbitol-to-Sweetness Series

Specimen SS-F-SWT-608 // SWEET · IFI-S

Sorbitol-to-Sweetness Series I-IV-B

Saccharum officinarum

Sugarcane

Tier ISorbitol-to-Sweetness Series

Target Trait

1.3×

fold-change

Sorbitol accumulation fold-change

Technical Specifications

SKU

SS-F-SWT-608

Genomic Edits

3

GC Content

45.2%

Construct Length

2,507 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

130

Pathway Edits

  • S6PDH sorbitol-6-phosphate dehydrogenase overexpression for direct conversion of glucose-6-P to sorbitol
  • SDH sorbitol dehydrogenase downregulation to prevent catabolism of accumulated sorbitol
  • SPS sucrose phosphate synthase activity optimization for sucrose-sorbitol metabolic balance
  • SPP sucrose phosphate phosphatase co-expression for enhanced sucrose-to-sorbitol flux management

Key Interventions

S6PDH overexpression for direct glucose-6-phosphate conversion to sorbitol in sugarcaneSDH downregulation preventing sorbitol catabolism to maintain high steady-state accumulationSPS co-optimization balancing sucrose-sorbitol carbon partitioning for desired sweetness profile

IFI Subscale Profile

IFI Profile

IFI-S
130
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: sorbitol accumulation research in sugarcane for polyol sweetener engineering and metabolic flux analysis with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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