IFI-S · Glycyrrhizin Series
Specimen SS-F-SWT-599 // SWEET · IFI-S
Glycyrrhizin Series V-IV-A
Stevia rebaudiana
Stevia
Tier VGlycyrrhizin Series
Target Trait
5×
fold-change
Glycyrrhizin fold-change
Technical Specifications
SKU
SS-F-SWT-599
Genomic Edits
9
GC Content
47.4%
Construct Length
4,758 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
500
Pathway Edits
- bAS beta-amyrin synthase overexpression initiating the pentacyclic triterpene scaffold in Stevia
- CYP88D6 cytochrome P450 integration for C-11 and C-30 oxidation steps toward glycyrrhetic acid
- CYP72A154 expression cassette for the C-30 carboxylation step in glycyrrhizin biosynthesis
- UGT73P2 glucuronosyltransferase integration for first glucuronidation step toward glycyrrhizin
- UGT91H4 expression for the second glucuronidation completing the glycyrrhizin disaccharide chain
- HMGR upregulation for expanded triterpenoid precursor supply via enhanced MVA pathway flux
- Competing flavonoid pathway suppression to redirect phenylpropanoid intermediates to triterpenoids
Key Interventions
bAS overexpression initiating pentacyclic triterpene scaffold for glycyrrhizin precursor in SteviaCYP88D6 and CYP72A154 sequential hydroxylation and carboxylation toward glycyrrhetic acidUGT73P2 and UGT91H4 dual glucuronosylation for complete glycyrrhizin disaccharide assembly
IFI Subscale Profile
IFI Profile
IFI-S500
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
industrial glycyrrhizin engineering for scalable licorice-flavor sweetener ingredient manufacturing
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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