Glycyrrhizin Series V-PR-B

Stevia rebaudiana

Stevia

Tier VGlycyrrhizin Series

Target Trait

5×

fold-change

Glycyrrhizin fold-change

Technical Specifications

SKU

SS-F-SWT-598

Genomic Edits

GC Content

45.3%

Construct Length

4,741 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

500

Pathway Edits

bAS beta-amyrin synthase overexpression initiating the pentacyclic triterpene scaffold in Stevia
CYP88D6 cytochrome P450 integration for C-11 and C-30 oxidation steps toward glycyrrhetic acid
CYP72A154 expression cassette for the C-30 carboxylation step in glycyrrhizin biosynthesis
UGT73P2 glucuronosyltransferase integration for first glucuronidation step toward glycyrrhizin
UGT91H4 expression for the second glucuronidation completing the glycyrrhizin disaccharide chain
HMGR upregulation for expanded triterpenoid precursor supply via enhanced MVA pathway flux
Competing flavonoid pathway suppression to redirect phenylpropanoid intermediates to triterpenoids

Key Interventions

bAS overexpression initiating pentacyclic triterpene scaffold for glycyrrhizin precursor in SteviaCYP88D6 and CYP72A154 sequential hydroxylation and carboxylation toward glycyrrhetic acidUGT73P2 and UGT91H4 dual glucuronosylation for complete glycyrrhizin disaccharide assembly

IFI Subscale Profile

IFI Profile

IFI-S

500

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: industrial glycyrrhizin engineering for scalable licorice-flavor sweetener ingredient manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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