IFI-S · Glycyrrhizin Series

Specimen SS-F-SWT-586 // SWEET · IFI-S

Glycyrrhizin Series IV-AG-B

Stevia rebaudiana

Stevia

Tier IVGlycyrrhizin Series

Target Trait

4.25×

fold-change

Glycyrrhizin fold-change

Technical Specifications

SKU

SS-F-SWT-586

Genomic Edits

8

GC Content

45.6%

Construct Length

4,326 bp

Promoter System

35S CaMV promoter

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R2/TAS1R3

IFI Score

425

Pathway Edits

  • bAS beta-amyrin synthase overexpression initiating the pentacyclic triterpene scaffold in Stevia
  • CYP88D6 cytochrome P450 integration for C-11 and C-30 oxidation steps toward glycyrrhetic acid
  • CYP72A154 expression cassette for the C-30 carboxylation step in glycyrrhizin biosynthesis
  • UGT73P2 glucuronosyltransferase integration for first glucuronidation step toward glycyrrhizin
  • UGT91H4 expression for the second glucuronidation completing the glycyrrhizin disaccharide chain
  • HMGR upregulation for expanded triterpenoid precursor supply via enhanced MVA pathway flux
  • Competing flavonoid pathway suppression to redirect phenylpropanoid intermediates to triterpenoids

Key Interventions

bAS overexpression initiating pentacyclic triterpene scaffold for glycyrrhizin precursor in SteviaCYP88D6 and CYP72A154 sequential hydroxylation and carboxylation toward glycyrrhetic acidUGT73P2 and UGT91H4 dual glucuronosylation for complete glycyrrhizin disaccharide assembly

IFI Subscale Profile

IFI Profile

IFI-S
425
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: regulatory metabolite profiling of engineered glycyrrhizin for clean-label functional sweetener submissions with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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