IFI-S · Glycyrrhizin Series

Specimen SS-F-SWT-576 // SWEET · IFI-S

Glycyrrhizin Series II-IV-B

Stevia rebaudiana

Stevia

Tier IIGlycyrrhizin Series

Target Trait

2.75×

fold-change

Glycyrrhizin fold-change

Technical Specifications

SKU

SS-F-SWT-576

Genomic Edits

5

GC Content

43.7%

Construct Length

3,154 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

275

Pathway Edits

  • bAS beta-amyrin synthase overexpression initiating the pentacyclic triterpene scaffold in Stevia
  • CYP88D6 cytochrome P450 integration for C-11 and C-30 oxidation steps toward glycyrrhetic acid
  • CYP72A154 expression cassette for the C-30 carboxylation step in glycyrrhizin biosynthesis
  • UGT73P2 glucuronosyltransferase integration for first glucuronidation step toward glycyrrhizin
  • UGT91H4 expression for the second glucuronidation completing the glycyrrhizin disaccharide chain

Key Interventions

bAS overexpression initiating pentacyclic triterpene scaffold for glycyrrhizin precursor in SteviaCYP88D6 and CYP72A154 sequential hydroxylation and carboxylation toward glycyrrhetic acidUGT73P2 and UGT91H4 dual glucuronosylation for complete glycyrrhizin disaccharide assembly

IFI Subscale Profile

IFI Profile

IFI-S
275
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: pilot-scale glycyrrhizin production in Stevia for natural licorice sweetener formulation testing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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