IFI-S · Glycyrrhizin Series
Specimen SS-F-SWT-567 // SWEET · IFI-S
Glycyrrhizin Series I-IV-A
Stevia rebaudiana
Stevia
Tier IGlycyrrhizin Series
Target Trait
2×
fold-change
Glycyrrhizin fold-change
Technical Specifications
SKU
SS-F-SWT-567
Genomic Edits
4
GC Content
46%
Construct Length
2,790 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
200
Pathway Edits
- bAS beta-amyrin synthase overexpression initiating the pentacyclic triterpene scaffold in Stevia
- CYP88D6 cytochrome P450 integration for C-11 and C-30 oxidation steps toward glycyrrhetic acid
- CYP72A154 expression cassette for the C-30 carboxylation step in glycyrrhizin biosynthesis
- UGT73P2 glucuronosyltransferase integration for first glucuronidation step toward glycyrrhizin
Key Interventions
bAS overexpression initiating pentacyclic triterpene scaffold for glycyrrhizin precursor in SteviaCYP88D6 and CYP72A154 sequential hydroxylation and carboxylation toward glycyrrhetic acidUGT73P2 and UGT91H4 dual glucuronosylation for complete glycyrrhizin disaccharide assembly
IFI Subscale Profile
IFI Profile
IFI-S200
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
glycyrrhizin pathway engineering research for heterologous triterpene sweet compound biosynthesis
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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