Miraculin Accumulation Series V-AG-B

Solanum lycopersicum

Tomato

Tier VMiraculin Accumulation Series

Target Trait

6.5

IFI score

Miraculin taste-modifying protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-554

Genomic Edits

GC Content

43.6%

Construct Length

4,413 bp

Promoter System

35S CaMV promoter

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R2/TAS1R3

IFI Score

650

Pathway Edits

miraculin-cDNA expression cassette for heterologous taste-modifying protein production in tomato
MIR-promoter fruit-specific regulatory element for miraculin accumulation in tomato fruit
Ext1 extensin signal peptide fusion for cell wall targeting and miraculin stability
N-glycosylation site optimization for proper miraculin homodimer assembly in tomato
Protease inhibitor co-expression for in planta protection of miraculin protein integrity
Fruit-ripening-linked promoter element for synchronized miraculin and lycopene accumulation
P19 silencing suppressor co-expression for amplified heterologous miraculin protein yield

Key Interventions

miraculin-cDNA integration under MIR fruit-specific promoter for taste-modifying protein accumulationExt1 signal peptide fusion for cell wall targeting enabling miraculin homodimer stabilityN-glycosylation site optimization for correct miraculin homodimer assembly and function

IFI Subscale Profile

IFI Profile

IFI-S

650

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: industrial miraculin production in tomato for scalable taste-conversion functional ingredient manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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