Specimen SS-F-SWT-552 // SWEET · IFI-S
Miraculin Accumulation Series IV-IV-B
Solanum lycopersicum
Tomato
Target Trait
5.5
IFI score
Miraculin taste-modifying protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-552
Genomic Edits
7
GC Content
45.9%
Construct Length
3,968 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
550
Pathway Edits
- miraculin-cDNA expression cassette for heterologous taste-modifying protein production in tomato
- MIR-promoter fruit-specific regulatory element for miraculin accumulation in tomato fruit
- Ext1 extensin signal peptide fusion for cell wall targeting and miraculin stability
- N-glycosylation site optimization for proper miraculin homodimer assembly in tomato
- Protease inhibitor co-expression for in planta protection of miraculin protein integrity
- Fruit-ripening-linked promoter element for synchronized miraculin and lycopene accumulation
- P19 silencing suppressor co-expression for amplified heterologous miraculin protein yield
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: regulatory documentation for miraculin as a natural taste-modifying sweet protein ingredient with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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