IFI-S · Miraculin Accumulation Series

Specimen SS-F-SWT-549 // SWEET · IFI-S

Miraculin Accumulation Series IV-PR-A

Solanum lycopersicum

Tomato

Tier IVMiraculin Accumulation Series

Target Trait

5.5

IFI score

Miraculin taste-modifying protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-549

Genomic Edits

7

GC Content

49.3%

Construct Length

3,917 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

550

Pathway Edits

  • miraculin-cDNA expression cassette for heterologous taste-modifying protein production in tomato
  • MIR-promoter fruit-specific regulatory element for miraculin accumulation in tomato fruit
  • Ext1 extensin signal peptide fusion for cell wall targeting and miraculin stability
  • N-glycosylation site optimization for proper miraculin homodimer assembly in tomato
  • Protease inhibitor co-expression for in planta protection of miraculin protein integrity
  • Fruit-ripening-linked promoter element for synchronized miraculin and lycopene accumulation
  • P19 silencing suppressor co-expression for amplified heterologous miraculin protein yield

Key Interventions

miraculin-cDNA integration under MIR fruit-specific promoter for taste-modifying protein accumulationExt1 signal peptide fusion for cell wall targeting enabling miraculin homodimer stabilityN-glycosylation site optimization for correct miraculin homodimer assembly and function

IFI Subscale Profile

IFI Profile

IFI-S
550
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

regulatory documentation for miraculin as a natural taste-modifying sweet protein ingredient

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Sweet · IFI-SAll Modalities