Specimen SS-F-SWT-544 // SWEET · IFI-S
Miraculin Accumulation Series III-IV-B
Solanum lycopersicum
Tomato
Target Trait
4.5
IFI score
Miraculin taste-modifying protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-544
Genomic Edits
6
GC Content
44.3%
Construct Length
3,621 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
450
Pathway Edits
- miraculin-cDNA expression cassette for heterologous taste-modifying protein production in tomato
- MIR-promoter fruit-specific regulatory element for miraculin accumulation in tomato fruit
- Ext1 extensin signal peptide fusion for cell wall targeting and miraculin stability
- N-glycosylation site optimization for proper miraculin homodimer assembly in tomato
- Protease inhibitor co-expression for in planta protection of miraculin protein integrity
- Fruit-ripening-linked promoter element for synchronized miraculin and lycopene accumulation
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: commercial miraculin accumulation platform for plant-derived taste modification ingredient supply with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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