IFI-S · Miraculin Accumulation Series

Specimen SS-F-SWT-536 // SWEET · IFI-S

Miraculin Accumulation Series II-IV-B

Solanum lycopersicum

Tomato

Tier IIMiraculin Accumulation Series

Target Trait

3.5

IFI score

Miraculin taste-modifying protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-536

Genomic Edits

5

GC Content

43.7%

Construct Length

3,274 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

350

Pathway Edits

  • miraculin-cDNA expression cassette for heterologous taste-modifying protein production in tomato
  • MIR-promoter fruit-specific regulatory element for miraculin accumulation in tomato fruit
  • Ext1 extensin signal peptide fusion for cell wall targeting and miraculin stability
  • N-glycosylation site optimization for proper miraculin homodimer assembly in tomato
  • Protease inhibitor co-expression for in planta protection of miraculin protein integrity

Key Interventions

miraculin-cDNA integration under MIR fruit-specific promoter for taste-modifying protein accumulationExt1 signal peptide fusion for cell wall targeting enabling miraculin homodimer stabilityN-glycosylation site optimization for correct miraculin homodimer assembly and function

IFI Subscale Profile

IFI Profile

IFI-S
350
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: pilot-scale miraculin production for sour-to-sweet taste conversion functional food applications with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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