IFI-S · Monellin Expression Series

Specimen SS-F-SWT-520 // SWEET · IFI-S

Monellin Expression Series V-IV-B

Solanum lycopersicum

Tomato

Tier VMonellin Expression Series

Target Trait

8

IFI score

Monellin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-520

Genomic Edits

8

GC Content

44.2%

Construct Length

4,435 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

800

Pathway Edits

  • scMonellin single-chain fused gene expression cassette for stable monellin accumulation in tomato
  • 35S enhancer-driven promoter element for constitutive scMonellin expression
  • PR1a secretory signal sequence for apoplastic targeting and enhanced monellin stability
  • Tobacco vacuolar targeting signal for subcellular compartmentalization strategy
  • P19 gene silencing suppressor co-expression for amplified scMonellin protein yield
  • ER-retention signal deletion variant for improved monellin secretory pathway transit
  • Heat-shock protein 70 chaperone co-expression for stabilized monellin folding in tomato

Key Interventions

scMonellin single-chain construct integration for thermostabilized monellin production in tomatoPR1a signal peptide fusion for apoplastic targeting and enhanced monellin stability35S enhancer-driven transcriptional amplification for maximum scMonellin expression output

IFI Subscale Profile

IFI Profile

IFI-S
800
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: industrial scMonellin production in tomato for scalable thermostable sweet protein manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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