IFI-S · Monellin Expression Series

Specimen SS-F-SWT-510 // SWEET · IFI-S

Monellin Expression Series IV-PR-B

Solanum lycopersicum

Tomato

Tier IVMonellin Expression Series

Target Trait

6.75

IFI score

Monellin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-510

Genomic Edits

7

GC Content

44.8%

Construct Length

4,054 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

675

Pathway Edits

  • scMonellin single-chain fused gene expression cassette for stable monellin accumulation in tomato
  • 35S enhancer-driven promoter element for constitutive scMonellin expression
  • PR1a secretory signal sequence for apoplastic targeting and enhanced monellin stability
  • Tobacco vacuolar targeting signal for subcellular compartmentalization strategy
  • P19 gene silencing suppressor co-expression for amplified scMonellin protein yield
  • ER-retention signal deletion variant for improved monellin secretory pathway transit
  • Heat-shock protein 70 chaperone co-expression for stabilized monellin folding in tomato

Key Interventions

scMonellin single-chain construct integration for thermostabilized monellin production in tomatoPR1a signal peptide fusion for apoplastic targeting and enhanced monellin stability35S enhancer-driven transcriptional amplification for maximum scMonellin expression output

IFI Subscale Profile

IFI Profile

IFI-S
675
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: regulatory sweet protein documentation for monellin-based natural sweetener ingredient submissions with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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