IFI-S · Monellin Expression Series

Specimen SS-F-SWT-503 // SWEET · IFI-S

Monellin Expression Series III-IV-A

Solanum lycopersicum

Tomato

Tier IIIMonellin Expression Series

Target Trait

5.5

IFI score

Monellin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-503

Genomic Edits

6

GC Content

48.9%

Construct Length

3,524 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

550

Pathway Edits

  • scMonellin single-chain fused gene expression cassette for stable monellin accumulation in tomato
  • 35S enhancer-driven promoter element for constitutive scMonellin expression
  • PR1a secretory signal sequence for apoplastic targeting and enhanced monellin stability
  • Tobacco vacuolar targeting signal for subcellular compartmentalization strategy
  • P19 gene silencing suppressor co-expression for amplified scMonellin protein yield
  • ER-retention signal deletion variant for improved monellin secretory pathway transit

Key Interventions

scMonellin single-chain construct integration for thermostabilized monellin production in tomatoPR1a signal peptide fusion for apoplastic targeting and enhanced monellin stability35S enhancer-driven transcriptional amplification for maximum scMonellin expression output

IFI Subscale Profile

IFI Profile

IFI-S
550
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

commercial scMonellin accumulation platform for plant-derived sweet protein ingredient supply chains

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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