IFI-S · Monellin Expression Series
Specimen SS-F-SWT-485 // SWEET · IFI-S
Monellin Expression Series I-PR-A
Solanum lycopersicum
Tomato
Tier IMonellin Expression Series
Target Trait
3
IFI score
Monellin sweet protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-485
Genomic Edits
4
GC Content
48.5%
Construct Length
2,796 bp
Promoter System
35S CaMV promoter
Expression System
PEG-mediated protoplast transfection
Host Strain
PEG/calcium transfection
Receptor Target
TAS1R2/TAS1R3
IFI Score
300
Pathway Edits
- scMonellin single-chain fused gene expression cassette for stable monellin accumulation in tomato
- 35S enhancer-driven promoter element for constitutive scMonellin expression
- PR1a secretory signal sequence for apoplastic targeting and enhanced monellin stability
- Tobacco vacuolar targeting signal for subcellular compartmentalization strategy
Key Interventions
scMonellin single-chain construct integration for thermostabilized monellin production in tomatoPR1a signal peptide fusion for apoplastic targeting and enhanced monellin stability35S enhancer-driven transcriptional amplification for maximum scMonellin expression output
IFI Subscale Profile
IFI Profile
IFI-S300
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
scMonellin expression validation for sweet protein research and stability engineering in tomato
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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