IFI-S · Brazzein Series

Specimen SS-F-SWT-472 // SWEET · IFI-S

Brazzein Series IV-IV-B

Capsicum annuum

Pepper

Tier IVBrazzein Series

Target Trait

7.63

IFI score

Brazzein sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-472

Genomic Edits

6

GC Content

45.9%

Construct Length

3,628 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

763

Pathway Edits

  • BRAZ codon-optimized expression cassette for heterologous brazzein production in Capsicum
  • Ubiquitin promoter integration for high-level constitutive brazzein expression
  • Vacuolar signal peptide fusion for protein sequestration and enhanced stability in planta
  • BiP chaperone co-expression for optimal brazzein disulfide bridge formation
  • mRNA 5-UTR optimization for enhanced ribosomal loading and translation efficiency
  • P19 gene silencing suppressor co-expression for elevated heterologous protein yield
  • 3-UTR polyadenylation signal optimization for extended brazzein transcript stability

Key Interventions

BRAZ codon-optimized transgene under ubiquitin promoter for high-yield brazzein accumulationVacuolar signal peptide fusion for protein stability and sequestration in Capsicum tissueBiP chaperone co-expression ensuring correct brazzein disulfide bridge folding architecture

IFI Subscale Profile

IFI Profile

IFI-S
763
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: regulatory documentation of brazzein sweet protein accumulation for clean-label sweetener filings with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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