IFI-S · Brazzein Series

Specimen SS-F-SWT-446 // SWEET · IFI-S

Brazzein Series I-PR-B

Capsicum annuum

Pepper

Tier IBrazzein Series

Target Trait

3.5

IFI score

Brazzein sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-446

Genomic Edits

3

GC Content

44.7%

Construct Length

2,353 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

350

Pathway Edits

  • BRAZ codon-optimized expression cassette for heterologous brazzein production in Capsicum
  • Ubiquitin promoter integration for high-level constitutive brazzein expression
  • Vacuolar signal peptide fusion for protein sequestration and enhanced stability in planta
  • BiP chaperone co-expression for optimal brazzein disulfide bridge formation

Key Interventions

BRAZ codon-optimized transgene under ubiquitin promoter for high-yield brazzein accumulationVacuolar signal peptide fusion for protein stability and sequestration in Capsicum tissueBiP chaperone co-expression ensuring correct brazzein disulfide bridge folding architecture

IFI Subscale Profile

IFI Profile

IFI-S
350
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: brazzein expression validation for small sweet protein research in Capsicum annuum pepper with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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