Thaumatin Expression Series V-IV-B

Capsicum annuum

Pepper

Tier VThaumatin Expression Series

Target Trait

IFI score

Thaumatin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-440

Genomic Edits

GC Content

44.2%

Construct Length

4,275 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

1,000

Pathway Edits

THAU-I codon-optimized expression cassette for high-level thaumatin I production in Capsicum
35S-promoter-driven element for constitutive thaumatin I accumulation in pepper tissue
Secretory signal peptide fusion for apoplastic targeting and enhanced thaumatin I stability
CaMV enhancer element insertion for amplified transcriptional output of thaumatin construct
Chaperone co-expression (PDI and BiP) for correct disulfide bridge formation in thaumatin I
Vacuolar targeting signal alternative for protein sequestration and in planta protection
mRNA 3-UTR stability element for extended thaumatin I transcript half-life and translation

Key Interventions

THAU-I codon-optimized transgene integration for high-yield thaumatin I accumulation in CapsicumSignal peptide engineering for optimal apoplastic secretion and protein folding fidelityCaMV enhancer-driven transcriptional amplification for maximum thaumatin I expression output

IFI Subscale Profile

IFI Profile

IFI-S

1,000

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: industrial thaumatin production system for scalable sweet protein ingredient manufacturing in pepper crops with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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