Specimen SS-F-SWT-438 // SWEET · IFI-S
Thaumatin Expression Series V-PR-B
Capsicum annuum
Pepper
Target Trait
10
IFI score
Thaumatin sweet protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-438
Genomic Edits
8
GC Content
45.3%
Construct Length
4,241 bp
Promoter System
35S CaMV promoter
Expression System
PEG-mediated protoplast transfection
Host Strain
PEG/calcium transfection
Receptor Target
TAS1R2/TAS1R3
IFI Score
1,000
Pathway Edits
- THAU-I codon-optimized expression cassette for high-level thaumatin I production in Capsicum
- 35S-promoter-driven element for constitutive thaumatin I accumulation in pepper tissue
- Secretory signal peptide fusion for apoplastic targeting and enhanced thaumatin I stability
- CaMV enhancer element insertion for amplified transcriptional output of thaumatin construct
- Chaperone co-expression (PDI and BiP) for correct disulfide bridge formation in thaumatin I
- Vacuolar targeting signal alternative for protein sequestration and in planta protection
- mRNA 3-UTR stability element for extended thaumatin I transcript half-life and translation
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: industrial thaumatin production system for scalable sweet protein ingredient manufacturing in pepper crops with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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