Specimen SS-F-SWT-432 // SWEET · IFI-S
Thaumatin Expression Series IV-IV-B
Capsicum annuum
Pepper
Target Trait
8.5
IFI score
Thaumatin sweet protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-432
Genomic Edits
7
GC Content
45.9%
Construct Length
3,928 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
850
Pathway Edits
- THAU-I codon-optimized expression cassette for high-level thaumatin I production in Capsicum
- 35S-promoter-driven element for constitutive thaumatin I accumulation in pepper tissue
- Secretory signal peptide fusion for apoplastic targeting and enhanced thaumatin I stability
- CaMV enhancer element insertion for amplified transcriptional output of thaumatin construct
- Chaperone co-expression (PDI and BiP) for correct disulfide bridge formation in thaumatin I
- Vacuolar targeting signal alternative for protein sequestration and in planta protection
- mRNA 3-UTR stability element for extended thaumatin I transcript half-life and translation
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: regulatory sweet protein documentation for thaumatin-based clean-label flavor enhancement applications with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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