IFI-S · Thaumatin Expression Series

Specimen SS-F-SWT-431 // SWEET · IFI-S

Thaumatin Expression Series IV-IV-A

Capsicum annuum

Pepper

Tier IVThaumatin Expression Series

Target Trait

8.5

IFI score

Thaumatin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-431

Genomic Edits

7

GC Content

46.2%

Construct Length

3,911 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

850

Pathway Edits

  • THAU-I codon-optimized expression cassette for high-level thaumatin I production in Capsicum
  • 35S-promoter-driven element for constitutive thaumatin I accumulation in pepper tissue
  • Secretory signal peptide fusion for apoplastic targeting and enhanced thaumatin I stability
  • CaMV enhancer element insertion for amplified transcriptional output of thaumatin construct
  • Chaperone co-expression (PDI and BiP) for correct disulfide bridge formation in thaumatin I
  • Vacuolar targeting signal alternative for protein sequestration and in planta protection
  • mRNA 3-UTR stability element for extended thaumatin I transcript half-life and translation

Key Interventions

THAU-I codon-optimized transgene integration for high-yield thaumatin I accumulation in CapsicumSignal peptide engineering for optimal apoplastic secretion and protein folding fidelityCaMV enhancer-driven transcriptional amplification for maximum thaumatin I expression output

IFI Subscale Profile

IFI Profile

IFI-S
850
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

regulatory sweet protein documentation for thaumatin-based clean-label flavor enhancement applications

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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