IFI-S · Mogroside V Series

Specimen SS-F-SWT-357 // SWEET · IFI-S

Mogroside V Series V-PR-A

Momordica grosvenorii

Monk Fruit

Tier VMogroside V Series

Target Trait

5.5×

fold-change

Mogroside V yield fold-change

Technical Specifications

SKU

SS-F-SWT-357

Genomic Edits

9

GC Content

46.9%

Construct Length

4,644 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

550

Pathway Edits

  • CYP87D18 overexpression for oxidative cyclization of 2,3-oxidosqualene to mogrol aglycone
  • UGT73A1 upregulation for the initial glucosylation step from mogrol to mogroside I
  • CYP450-M1 expression enhancement for oxidative functionalization during mogroside pathway
  • SQS squalene synthase upregulation for increased triterpenoid precursor squalene flux
  • SE squalene epoxidase activity enhancement for 2,3-oxidosqualene availability
  • GT1 glucosyltransferase integration for multi-step glucosylation cascade toward mogroside V
  • Competing cucurbitane pathway suppression to redirect flux toward mogrosides

Key Interventions

CYP87D18-mediated oxidative cyclization for mogrol aglycone biosynthesis in MomordicaUGT73A1-driven initial glucosylation establishing the mogroside glycosylation cascadeSQS and SE co-upregulation expanding triterpenoid precursor pool for mogroside V synthesis

IFI Subscale Profile

IFI Profile

IFI-S
550
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

industrial mogroside V engineering for high-purity monk fruit sweetener manufacturing scale-up

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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