Stevioside Optimization Series V-IV-B

Stevia rebaudiana

Stevia

Tier VStevioside Optimization Series

Target Trait

4×

fold-change

Stevioside profile fold-change

Technical Specifications

SKU

SS-F-SWT-320

Genomic Edits

GC Content

44.2%

Construct Length

4,435 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

400

Pathway Edits

KAH cytochrome P450 optimization for improved ent-kaurenoic acid hydroxylation efficiency
UGT85C2 upregulation for the first glucosylation step of steviol to steviolmonoside
UGT74G1 expression enhancement for C-19 glucosylation toward stevioside completion
CPS copalyl diphosphate synthase upregulation for expanded diterpene pathway flux
KO ent-kaurene oxidase activity enhancement for increased steviol biosynthetic rate
Rebaudioside diversion pathway tuning to maintain optimal stevioside-to-RebA ratio
Transcription factor SrDRW1 overexpression for pathway-wide regulatory enhancement

Key Interventions

KAH and KO co-optimization to increase net steviol production rate in SteviaUGT85C2 and UGT74G1 sequential upregulation for complete stevioside glycosylationCPS-driven diterpene flux expansion to support higher stevioside accumulation

IFI Subscale Profile

IFI Profile

IFI-S

400

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: industrial stevioside engineering for cost-optimized sweetener production with defined sweetness profile with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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