IFI-S · Stevioside Optimization Series
Specimen SS-F-SWT-313 // SWEET · IFI-S
Stevioside Optimization Series V-AG-A
Stevia rebaudiana
Stevia
Tier VStevioside Optimization Series
Target Trait
4×
fold-change
Stevioside profile fold-change
Technical Specifications
SKU
SS-F-SWT-313
Genomic Edits
8
GC Content
47.5%
Construct Length
4,316 bp
Promoter System
35S CaMV promoter
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TAS1R2/TAS1R3
IFI Score
400
Pathway Edits
- KAH cytochrome P450 optimization for improved ent-kaurenoic acid hydroxylation efficiency
- UGT85C2 upregulation for the first glucosylation step of steviol to steviolmonoside
- UGT74G1 expression enhancement for C-19 glucosylation toward stevioside completion
- CPS copalyl diphosphate synthase upregulation for expanded diterpene pathway flux
- KO ent-kaurene oxidase activity enhancement for increased steviol biosynthetic rate
- Rebaudioside diversion pathway tuning to maintain optimal stevioside-to-RebA ratio
- Transcription factor SrDRW1 overexpression for pathway-wide regulatory enhancement
Key Interventions
KAH and KO co-optimization to increase net steviol production rate in SteviaUGT85C2 and UGT74G1 sequential upregulation for complete stevioside glycosylationCPS-driven diterpene flux expansion to support higher stevioside accumulation
IFI Subscale Profile
IFI Profile
IFI-S400
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
industrial stevioside engineering for cost-optimized sweetener production with defined sweetness profile
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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