IFI-S · Stevioside Optimization Series

Specimen SS-F-SWT-312 // SWEET · IFI-S

Stevioside Optimization Series IV-IV-B

Stevia rebaudiana

Stevia

Tier IVStevioside Optimization Series

Target Trait

3.38×

fold-change

Stevioside profile fold-change

Technical Specifications

SKU

SS-F-SWT-312

Genomic Edits

7

GC Content

45.9%

Construct Length

3,888 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

338

Pathway Edits

  • KAH cytochrome P450 optimization for improved ent-kaurenoic acid hydroxylation efficiency
  • UGT85C2 upregulation for the first glucosylation step of steviol to steviolmonoside
  • UGT74G1 expression enhancement for C-19 glucosylation toward stevioside completion
  • CPS copalyl diphosphate synthase upregulation for expanded diterpene pathway flux
  • KO ent-kaurene oxidase activity enhancement for increased steviol biosynthetic rate
  • Rebaudioside diversion pathway tuning to maintain optimal stevioside-to-RebA ratio
  • Transcription factor SrDRW1 overexpression for pathway-wide regulatory enhancement

Key Interventions

KAH and KO co-optimization to increase net steviol production rate in SteviaUGT85C2 and UGT74G1 sequential upregulation for complete stevioside glycosylationCPS-driven diterpene flux expansion to support higher stevioside accumulation

IFI Subscale Profile

IFI Profile

IFI-S
338
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: regulatory metabolite profiling for stevioside-focused clean-label ingredient submissions with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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