IFI-S · Rebaudioside M Series

Specimen SS-F-SWT-280 // SWEET · IFI-S

Rebaudioside M Series V-IV-B

Stevia rebaudiana

Stevia

Tier VRebaudioside M Series

Target Trait

8×

fold-change

RebM yield fold-change

Technical Specifications

SKU

SS-F-SWT-280

Genomic Edits

11

GC Content

44.2%

Construct Length

5,495 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

800

Pathway Edits

  • UGT91D2 expression cassette for multi-glucosylation of RebD intermediate to rebaudioside M
  • UGT40 upregulation for the final C-3' glucosylation step in RebM biosynthesis
  • UGT76G1 co-expression to maintain upstream RebA and RebD intermediate supply
  • UGT74G1 expression enhancement for complete C-19 glucosylation toward RebM precursors
  • CDPG co-expression for enhanced ent-copalyl diphosphate precursor availability
  • Antisense UGT85C2 element suppressing stevioside diversion and maximizing RebM-directed flux
  • RebD intermediate stabilization via transient vacuolar targeting signal deletion
  • Transcriptional enhancer element insertion upstream of UGT91D2 locus for elevated expression

Key Interventions

UGT91D2 and UGT40 dual overexpression for complete RebM multi-glucosylation cascadeUGT76G1 co-expression maintaining upstream RebA and RebD intermediate fluxStevioside diversion pathway suppression to maximize RebM-directed carbon flow

IFI Subscale Profile

IFI Profile

IFI-S
800
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: industrial RebM production optimization for high-volume reduced-sugar and functional food manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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