IFI-S · Rebaudioside M Series
Specimen SS-F-SWT-279 // SWEET · IFI-S
Rebaudioside M Series V-IV-A
Stevia rebaudiana
Stevia
Tier VRebaudioside M Series
Target Trait
8×
fold-change
RebM yield fold-change
Technical Specifications
SKU
SS-F-SWT-279
Genomic Edits
11
GC Content
47.4%
Construct Length
5,478 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
800
Pathway Edits
- UGT91D2 expression cassette for multi-glucosylation of RebD intermediate to rebaudioside M
- UGT40 upregulation for the final C-3' glucosylation step in RebM biosynthesis
- UGT76G1 co-expression to maintain upstream RebA and RebD intermediate supply
- UGT74G1 expression enhancement for complete C-19 glucosylation toward RebM precursors
- CDPG co-expression for enhanced ent-copalyl diphosphate precursor availability
- Antisense UGT85C2 element suppressing stevioside diversion and maximizing RebM-directed flux
- RebD intermediate stabilization via transient vacuolar targeting signal deletion
- Transcriptional enhancer element insertion upstream of UGT91D2 locus for elevated expression
Key Interventions
UGT91D2 and UGT40 dual overexpression for complete RebM multi-glucosylation cascadeUGT76G1 co-expression maintaining upstream RebA and RebD intermediate fluxStevioside diversion pathway suppression to maximize RebM-directed carbon flow
IFI Subscale Profile
IFI Profile
IFI-S800
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
industrial RebM production optimization for high-volume reduced-sugar and functional food manufacturing
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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