IFI-S · Rebaudioside M Series

Specimen SS-F-SWT-255 // SWEET · IFI-S

Rebaudioside M Series II-IV-A

Stevia rebaudiana

Stevia

Tier IIRebaudioside M Series

Target Trait

4.25×

fold-change

RebM yield fold-change

Technical Specifications

SKU

SS-F-SWT-255

Genomic Edits

7

GC Content

47.3%

Construct Length

4,057 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

425

Pathway Edits

  • UGT91D2 expression cassette for multi-glucosylation of RebD intermediate to rebaudioside M
  • UGT40 upregulation for the final C-3' glucosylation step in RebM biosynthesis
  • UGT76G1 co-expression to maintain upstream RebA and RebD intermediate supply
  • UGT74G1 expression enhancement for complete C-19 glucosylation toward RebM precursors
  • CDPG co-expression for enhanced ent-copalyl diphosphate precursor availability

Key Interventions

UGT91D2 and UGT40 dual overexpression for complete RebM multi-glucosylation cascadeUGT76G1 co-expression maintaining upstream RebA and RebD intermediate fluxStevioside diversion pathway suppression to maximize RebM-directed carbon flow

IFI Subscale Profile

IFI Profile

IFI-S
425
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

pilot-scale RebM production for superior-sweetness beverage and confectionery formulation trials

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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