IFI-S · Rebaudioside M Series
Specimen SS-F-SWT-251 // SWEET · IFI-S
Rebaudioside M Series II-BL-A
Stevia rebaudiana
Stevia
Tier IIRebaudioside M Series
Target Trait
4.25×
fold-change
RebM yield fold-change
Technical Specifications
SKU
SS-F-SWT-251
Genomic Edits
7
GC Content
49.1%
Construct Length
3,989 bp
Promoter System
Ubiquitin/35S CaMV promoter
Expression System
Biolistic particle bombardment
Host Strain
Gold particle He-bombardment
Receptor Target
TAS1R2/TAS1R3
IFI Score
425
Pathway Edits
- UGT91D2 expression cassette for multi-glucosylation of RebD intermediate to rebaudioside M
- UGT40 upregulation for the final C-3' glucosylation step in RebM biosynthesis
- UGT76G1 co-expression to maintain upstream RebA and RebD intermediate supply
- UGT74G1 expression enhancement for complete C-19 glucosylation toward RebM precursors
- CDPG co-expression for enhanced ent-copalyl diphosphate precursor availability
Key Interventions
UGT91D2 and UGT40 dual overexpression for complete RebM multi-glucosylation cascadeUGT76G1 co-expression maintaining upstream RebA and RebD intermediate fluxStevioside diversion pathway suppression to maximize RebM-directed carbon flow
IFI Subscale Profile
IFI Profile
IFI-S425
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
pilot-scale RebM production for superior-sweetness beverage and confectionery formulation trials
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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